Chromotek/U2OS-CCC-TagRFP/代理商現貨

 螞蟻淘介紹：

螞蟻淘，【*極速】生物醫(yī)學科研用品輕松購

螞蟻淘(ebiomall）是一家生命科學領域的垂直電商平臺，公司專注生物醫(yī)學科研用品的  導購和品牌推廣，為海外廠方與國內代理經銷商搭建跨境貿易系統(tǒng)和交流平臺。

    產品齊全，200+分類，1000+品牌，500萬+產品信息。**，專業(yè)售后，到貨快，在線下單， 簡單輕松，節(jié)省科學家寶貴的科研時間。

借助互聯網平等而開放的力量，矢志實現生命科學領域的信息同步，我們相信一群充滿夢想的人，可以憑借著專業(yè)與執(zhí)著，讓你的購物更輕松！

Chromotek/U2OS-CCC-TagRFP/代理商現貨

    Description

　　U2OS-Cell Cycle Chromobody®-TagRFP

　　U2OS cell line stably expressing Cell Cycle Chromobody® fused to the red fluorescent protein TagRFP

　　Specificity

　　VHH antibody fragment against cell cycle marker PCNA (proliferating cell nuclear antigen)Actin-Chromobody®

　　Live cell imaging of U2OS-Cell Cycle Chromobody®-TagRFP

　　For live cell imaging cells were seeded in ibidi µ-Slides. Time series was acquired on a spinning disk microscope (UltraVIEW VoX, PerkinElmer) equipped with a 63x/1.4 NA Plan-Apochromat oil immersion objective and a humidified, temperature- and CO2-controlled live cell chamber. TagRFP was excited with a 561 nm laser-line for typically between 100-500 ms/ image with laser intensity set to max. 10%. Confocal image z-stacks of living cells were recorded with a frame size of 1000x1000 pixels, a pixel size of 100 nm and a z-step size of 400 nm every 30 minutes. Images shown are maximum intensity projections of few mid-z-sections.